Potentiated sera and process of making them.



'8 coma albumin, etc. For

" is introduced into found in the blood I f serum of such an animal is now able to hydrolyze carcinoma albumin as well as other" constituents of carcinomatous tissues.

M placing lyzer and allowing it to actupon carcinoma- 'QFFKGE.

EMIL ABDERHALDEN, 0F HALLE-ON-THE-SAALE, GERMANY.

POTENTIATED SERA AND PBOCESSOF MAKING THEM.

mana e.

No Drawing.

invented new and useful Improvements 1n ,Potentiated Sera and Process of Making Them, of which the following is a specification.

This invention relates to new sera and to new methods for making them.

It comprises as, new products, serav or animal fluids having a greatly'augmented power for'destroying or'lysing (dissolving) certain proteins such as carclnoma albumin,

sarcoma albumin, etc., and capable, .when injected into an animal. aifiicted with carcinoma, sarcoma, or certain infectious diseases, of manifesting thereon a distinct curative effect. 1

By my experimentsI have demonstrated that after the introduction of complex substances foreign to the blood, ferments ap- 2 pear in the blood plasma which are able to hydrolyze these complex substances. This observation has been utilized by me for the purpose of producing artificial protective ferments against carcinoma albumin, sarinstance, carcinoma tissue or an extract or autolysate of such tissue is injected under the skinof a dog,

and 2 to 5 days after the injection the blood 1 plasma of the animal is capable of splitting carcinoma albumin. This observation has been utilized for the preparation of antisera against carcinoma, sarcoma, and certain ini -fectious diseases. I

For the purpose of describing mypresent 'fi fi invention, I call the "products hitherto known: one animal sera. My potentiatedl sera are made from these one animal sera as described below in greater detail. I

into the abdomliial cavity or under after 2 to 5 days protective ferments are plasma, The .blood The resence of the ferments is determined by the serum of the animal in a Specification a Letters Patent.

citizen of the German Empire, resid-- acquires the same animal to animal or from one i If, 'for instance, carcinoma tissue is ini5 jected I the skin of the animal, or an extract of such a tissue or an autolysate or the expressed juice the blood channels, thennamely, that which ,additional amounts of the Patented Aug. 24L, 1915.

- Application filed May 14, 1915. Serial No. 28,195.

tous tissue, and then examining the dialyzed material for cleavage products of albumin according to the customary methods. Furthermore, throughthe action of acid upon carcinoma tissue, peptones can be prepared,

and these, together with the corresponding 1 serum, can be polarized. Continued observation at 37 deg. shows that the .rotary power of the mixture is permanently changed. If for such experiments serum from a normal animal which has not been injected with carcinomatous tissue be used, then no spl1tti ng up of the constituents of the carclnoma tissue takes place. The same applies to any given substratum, microorganisms, etc. Now I have made the astonishing discovery that if serum is taken from an animal thus treated and injected into'another animal, the serum of the second test animal properties, but in ,an enhanced degree. By repeated or successive transference of protective human being to another, the proteolytic action of the thus obtained potentiated serum in contact with certain substrata is augmented to an extraordinary extent. These the possibility for the first time of prepar ing potentiated sera which are rich in certain protective ferments. As has already been "demonstrated by many practical ex periments, it is poss'ble with the aid of such be obtained is also of the greatest'importance for the reasonthat it becomes possible I in this way to exclude any danger of an im fection through the serum of an animal that has been d1- ,rectly injected with tumor material might still contain living cells or microiirgamsms.

The new procedure affords forth first time the possibility of preparing curative sera for cancer, etc., one large scale; for by the only method of preparation hitherto known, consisting in a single injection, but a limited amount of serum was, obtainable,

could be secured For the production of one animal serum hitherto known other animals had again to be injected with freshtumor material, which (especially in the case of mterthe injected animal.

ferments from i observations offer .85 I

The fact that. pr t ti ierments can the injected materiaLfor. r

from

' toxin is formed and the hanced by repeated injections into new test p animals.

' uterine carcinoma,

45. "tissue to be injected can be is capable of splitting carcinoma tissue as inof infectious diseases is nal tumors) is diificult to secure. The new procedure, however, permits of an injection of a large number of other animals with material derived from the one first injected, and thus practically unlimited amounts of serum of increased potency can be obtained.

The potentiated serum for the treatment also obtained from the hitherto known one animal serum. In this case the one animal serumis drawn from the first animal already at the end of 3 to 5 days. Such a serum tectiveferments whereas in the ordinary serum which has been drawn from the animal after a period of 5 days these ferments have disappeared. After ferments appear to be eliminated or inactivated.- In view of this unexpected and pecular actionof the organism in causing the appearance and soon afterward the disappearance of protective ferments, it is necessary to maintain a care- .ful time-control of the process, and it is often desirable to withdraw the serum from '25" each animal in its turn when the protective ferment content of the serum in such animal has about reached a maximum. The one animal diphtheria serum or the one 'animal tuberculosis serum is then potentiated by repeated injections from animal-into animal until the desired strength in protective ferments is obtained. The one animal serum after being injected into the second animal is drawn from this end of 12 to 24 hours. Each subsequent serum exhibits the same characteristic increase of action-which can be further en- Example: The carcinoma, for instance, which must be completely fresh, is chopped up fine, is injected into a'normal horse, either under the skin or into the abdominal cavity, or even into the blood vessels.

previously submitted to autolysis "(autodigestion)- in the jected to strong pressure and the fluidj'thus obtained used for injection. At the end of 5 days, at the earliest after 2 days, blood is 'dicated bythe production of. dialyzable substances when it is allowed to react with said tissue. Such a serum, responding positively j to the abovetest, suitably filtered through a 6'0 Chamberland filter is injected into a second normal horse or into some other-animal.

7 .After 3 days blood is withdrawn from the animal and its serum a moremarked cleavage power. By further will be found to exhibit inoculations a serum very rich in protective erments can be prepared. This potentiated still contains prothat time the antianimal at the living cells and microor and this material The carcinoma ing an increased proportion fluid containing protective ferments induced by a protein normally foreign to said animal fluid. g I

3. A potentiated serum containing protective ferments and capable of reacting with. a cancerous tissue; to produce a substantially greater proportion of dialyzable substances than can be produced by a one animal serum reacting with said protein.

4. A potentiated serum obtainable from a one animal serum by injection thereof into, and withdrawal from, at least one other animal, and characterized by being rich in protective ferments, being free from living cells and microorganisms, and exhibiting a powerful proteolytic action in contact with cancerous tissue. a

5. A potentiated serum obtainable from a one animal serum by injectlon thereof rich in protective ferments, being free from anisms, and exhibiting a-powerful proteo ytic action in contact with carcinomatous tissue.

6. The process of producing a potentiated serum which comprises injecting a serum containing protectlve ferments specific to a protein foreign to the serum obtained from oneanimalinto asecond animal, and then withdrawing serum containing an increased proportion of such ferments. from said second animal, substantially as described.

7. The process of producing a potentiated serum which comprises successively transferring =serum containing protective ferbemgs suffering with containing prothe serum to pro- .100 into, and withdrawal from, at least one other animal, and-characterized by being ments specific to a prote nforeign to the serum from one animal to another through a series of animals and withdrawing serum containing an increased proportion of such erments from 'the'last animal of the series, substantially ias described. 3 8. The process of producinga potentiated serum which comprises injecting'into an animal a serum, containing ments and capable of exerting a proteolytlc protective feraction when in contact with cancerous tissue, and then withdrawing serum contajnof prote0t1v6 ferments from said animal, substantially as described.

9. The process of producing a potentiated serum which comprises injecting into an animal a serum, containing protective ferments and capable of exerting a proteolytic action when in contact with carcinomatous tissue, and then withdrawing serum containing an increased proportion of' protective ferments from said animal, substantially as described.

10. The process of producing a potentiated serum which comprises successively transferring serum containing protective ferments from one animal to another through a series of animals and Withdrawing serum from the last animal of the series, each withdrawal being made when the protective ferment content of the serum in the respective animal is at about a maximum, substantially as described.

11. A process of producing a serum rich in protective ferments which comprises injecting cancerous tissue into a test animal to induce the formation of protective ferments therein withdrawing serum from said animal before the protective ferments have disappeared, injecting such serum into a second animal to induce the production of a serum containing a greater proportion of protective ferments and repeating the Withdrawal and injection into fresh animals until a serum of the desired strength is obtained, substantially as described.

p In testimony whereof I have hereunto set my hand in the presence of two subscribing witnesses.

EMIL ABDERHALDEN.

\Vitnesses RUDOLPH FRICKE, WM. P. KENT. 

